Plasmid Vector - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | G90455 | |
| Name | pTM1290 | |
| Description | Multiplex CRISPR/Cas9 knockout system CRISPR final destination vector | |
| Accession | ||
| DSC-ID | ||
| Associated Gene(s) | ||
| Backbone Plasmid | pBluescript II | |
| Antibiotic Resistance | ampr neor | |
| References |
Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium. Sci Rep (2018) 8(1) 8471 [PubMed ID = 29855514] [RRC reference] |
|
| Map | Multiplex_CRISPR-Cas9_Assembly_System.pdf | |
| Sequence | pTM1290.txt | |
| Depositor | Tetsuya Muramoto | |
| Position | ||
| Source | Depositor | |
| Other Information | Ideal for multiple gene knockouts See Fig. S3 for details of the vectors. The multiple tRNA-sgRNA modules of the second module vectors can be assembled into the pTM1290 vector by BsmBI Golden Gate reaction. The combined Multiple tRNA-sgRNA modules can be digested with XhoI and HindIII and then cloned into pTM1285 or appropriate CRISPR vector. | |
| Use Condition | B1 | |
