Plasmid Vector - 詳細
| Request |
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|---|---|---|
| NBRP ID | G90484 | |
| Name | pTM1331 | |
| Description | All-in-one CRISPR/Cas9 vector Cas9 nickase-NLS-GFP, dual-tRNA-sgRNA (BpiI+Esp3I) Transient expression (Neo) | |
| Accession | ||
| DSC-ID | ||
| Associated Gene(s) | Cas9 nickase, D10A mutant of SpCas9 | |
| Backbone Plasmid | pBluescript II | |
| Antibiotic Resistance | ampr neor | |
| References |
Iriki H, Kawata T, Muramoto T.
Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase. PLoS One (2019) 14(10) e0224128 [PubMed ID = 31622451] [RRC文献情報] |
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| Map |
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|
| Sequence | pTM1331.txt | |
| Depositor | Tetsuya Muramoto | |
| Position | ||
| Source | Tetsuya Muramoto | |
| Other Information | Ideal for gene knockout and large deletion Lower off-target A dual sgRNA expression vector that allows single-step cloning is available as pTM1544. | |
| Use Condition | B1 | |
