Plasmid Vector - 詳細
NBRP ID | G90485 | |
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Name | pTM1355 | |
Description | All-in-one CRISPR/Cas9 vector Cas9 nickase-NLS-GFP, tRNA-sgRNA (Esp3I) Stable expression (Neo) | |
Accession | ||
DSC-ID | ||
Associated Gene(s) | Cas9 nickase, D10A mutant of SpCas9 | |
Backbone Plasmid | pDM1208 | |
Antibiotic Resistance | ampr neor | |
References |
Iriki H, Kawata T, Muramoto T.
Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase. PLoS One (2019) 14(10) e0224128 [PubMed ID = 31622451] [RRC文献情報] |
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Map | ||
Sequence | pTM1355.txt | |
Depositor | Tetsuya Muramoto | |
Source | Tetsuya Muramoto | |
Other Information | Ideal for gene knockout and large deletion Lower off-target Two sgRNA sequences need to be cloned separately into the vector | |
Use Condition | B1 |