Plasmid Vector - 詳細

NBRP ID G90485
Name pTM1355
Description All-in-one CRISPR/Cas9 vector Cas9 nickase-NLS-GFP, tRNA-sgRNA (Esp3I) Stable expression (Neo)
Accession
DSC-ID
Associated Gene(s) Cas9 nickase, D10A mutant of SpCas9 
Backbone Plasmid pDM1208
Antibiotic Resistance ampr  neor 
References Iriki H, Kawata T, Muramoto T.
Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase.
PLoS One  (2019)  14(10)   e0224128  
[PubMed ID = 31622451]  [RRC文献情報
Map
Sequence pTM1355.txt
Depositor Tetsuya Muramoto
Source Tetsuya Muramoto
Other Information Ideal for gene knockout and large deletion Lower off-target Two sgRNA sequences need to be cloned separately into the vector
Use Condition B1
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