Plasmid Vector - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | G90487 | |
| Name | pTM1544 | |
| Description | All-in-one CRISPR/Cas9 vector Cas9 nickase-NLS-GFP, dual-tRNA-sgRNA (BpiI) Transient expression (Neo) | |
| Accession | ||
| DSC-ID | ||
| Associated Gene(s) | Cas9 nickase, D10A mutant of SpCas9 | |
| Backbone Plasmid | pBluescript II | |
| Antibiotic Resistance | ampr neor | |
| References |
Iriki H, Kawata T, Muramoto T.
Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase. PLoS One (2019) 14(10) e0224128 [PubMed ID = 31622451] [RRC reference] |
|
| Map |
 |
|
| Sequence | pTM1544.txt | |
| Depositor | Tetsuya Muramoto | |
| Position | ||
| Source | Tetsuya Muramoto | |
| Other Information | Ideal for large deletion or knockouts Lower off-target Single-step cloning of dual sgRNAs | |
| Use Condition | B1 | |
