Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00008 | |
| Strain Name | gefT- | |
| Strain Summary | knockout of candidate cGMP-binding protein GbpC containing RasGEF domain; blasticidin resistant | |
| Strain Descriptor | gbpC- | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pcr pruducts with selection cassette | |
| Associated Gene | gbpC | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1, bsR,gbpC-[gbpC-bsr],pyr5-6-[pRG24],ura- | |
| Phenotype | axenic, blasticidin resistant, uracil auxotroph | |
| Axenic Growth | Y | |
| Viable Spore Formation | ||
| References |
Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium. Proc Natl Acad Sci U S A (2002) 99(10) 6749-54 [PubMed ID = 12011437] [RRC reference] Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ. A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. EMBO J (2002) 21(17) 4560-70 [PubMed ID = 12198158] [RRC reference] Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ. RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium. J Cell Sci (2005) 118(Pt 9) 1899-910 [PubMed ID = 15827084] [RRC reference] |
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| Other Information | originally deposited by Peter van Haastert to Dicty Stock Center | |
| Depositor | DSC | |
| Position | ||
| Source | P. Haastert | |
| Use Condition | A | |
| DSC | DBS0235995 | |
| Remarks | ||
| Images | ||
| Sequence | ||
