株リソース - 詳細
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| NBRP ID | S00143 | |
| Strain Name | pde6-, pdeE- | |
| Strain Summary | gbpB null mutant | |
| Strain Descriptor | gbpB- | |
| Systematic Name | DBS0235993 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pcr pruducts with selection cassette | |
| Associated Gene | pdeE | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pRG24],pdeE--[gbpB-pyr],ura+ | |
| Phenotype | axenic, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Kuwayama H, Kubohara Y.
Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis. PLoS One (2009) 4(8) e6658 [PubMed ID = 19684855] [RRC文献情報] Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL. Identification of four candidate cGMP targets in Dictyostelium. Proc Natl Acad Sci U S A (2002) 99(10) 6749-54 [PubMed ID = 12011437] [RRC文献情報] Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ. A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. EMBO J (2002) 21(17) 4560-70 [PubMed ID = 12198158] [RRC文献情報] Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ. RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium. J Cell Sci (2005) 118(Pt 9) 1899-910 [PubMed ID = 15827084] [RRC文献情報] Kubohara Y, Kikuchi H, Nguyen VH, Kuwayama H, Oshima Y. Evidence that differentiation-inducing factor-1 controls chemotaxis and cell differentiation, at least in part, via mitochondria in <i>D. discoideum</i>. Biol Open (2017) 6(6) 741-751 [PubMed ID = 28619991] [RRC文献情報] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | P. Haastert | |
| Use Condition | A | |
| DSC | DBS0235993 | |
| Remarks | ||
| Images | ||
| Sequence | ||
