Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00152 | |
| Strain Name | DH1 | |
| Strain Summary | uracil auxotroph; axenic | |
| Strain Descriptor | ||
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Other | |
| Parental Strain | AX3 (DBS0235542) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous deletion, pRG24 + FOA selection | |
| Associated Gene | pyr56 | |
| Foreign Gene | ||
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura- | |
| Phenotype | uracil auxotroph, axenic | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Caterina MJ, Milne JL, Devreotes PN.
Mutation of the third intracellular loop of the cAMP receptor, cAR1, of Dictyostelium yields mutants impaired in multiple signaling pathways. J Biol Chem (1994) 269(2) 1523-32 [PubMed ID = 8288619] [RRC reference] |
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| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | DSC | |
| Use Condition | A | |
| DSC | DBS0235696 | |
| Remarks | ||
| Images | ||
| Sequence | ||
