株リソース - 詳細
| Request |
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|
|---|---|---|
| NBRP ID | S00155 | |
| Strain Name | HL330 | |
| Strain Summary | Bill Loomis lab strain; axenic; uracil auxotroph | |
| Strain Descriptor | HL330 | |
| Systematic Name | HL330 | |
| Species | Dictyostelium discoideum | |
| Category | Other | |
| Parental Strain | AX4 (DBS0235551) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous deletion, pDIV1 (BglII fragment of pdeltaPYR + FOA selection) | |
| Associated Gene | pyr56 | |
| Foreign Gene | ||
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pdeltaPYR-BglII], ura- | |
| Phenotype | axenic, uracil auxotroph | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Kuspa A, Loomis WF.
Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. Proc Natl Acad Sci U S A (1992) 89(18) 8803-7 [PubMed ID = 1326764] [RRC文献情報] |
|
| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | A. Kuspa | |
| Use Condition | A | |
| DSC | DBS0236231 | |
| Remarks | ||
| Images | ||
| Sequence | ||
