株リソース - 詳細
Request |
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NBRP ID | S00244 | |
Strain Name | FCL-AA17(DdFHa-) | |
Strain Summary | DdFHa null mutant; axenic; resistant to blasticidin; axenic | |
Strain Descriptor | ||
Systematic Name | ||
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | KAX3 | |
Mutagenesis Method | homologous recombination | |
Mutagenesis Detail | the 5' (1530 nt) fragment of DdFHa cDNA was excised by digestion of FCLAA17 with Kpn I-Sma I, and then inserted into the Bam HI site of pBsrΔBam (Adachi et al., 1994) in the sense orientation. The 3' (531-1313 nt) fragment was excised by Kpn I-Bam HI dig | |
Associated Gene | DdFHa fhbA | |
Foreign Gene | bsR | |
Genotype | ||
Phenotype | ||
Axenic Growth | Y | |
Viable Spore Formation | OK | |
References |
Iijima M, Shimizu H, Tanaka Y, Urushihara H.
Identification and characterization of two flavohemoglobin genes in Dictyostelium discoideum. Cell Struct Funct (2000) 25(1) 47-55 [PubMed ID = 10791894] [RRC文献情報] |
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Other Information | ||
Depositor | Hideko Urushihara | |
Position | ||
Source | depositor | |
Use Condition | B1 | |
DSC | ||
Remarks | ||
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