Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00262 | |
| Strain Name | GAK-12 | |
| Strain Summary | gmsA null mutant in KAX3; resistant to blasticidin; axenic | |
| Strain Descriptor | KAX3/gmsA- | |
| Systematic Name | TM06K-02 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | KAX3 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pGEM-T | |
| Associated Gene | gmsA | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1, bsr, gmsA- | |
| Phenotype | axenic, blasticidin resistant, reduced fusion | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Muramoto T, Suzuki K, Shimizu H, Kohara Y, Kohriki E, Obara S, Tanaka Y, Urushihara H.
Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum. Mech Dev (2003) 120(8) 965-75 [PubMed ID = 12963116] [RRC reference] |
|
| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | T. Muramoto | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
