Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00339 | |
| Strain Name | gbpA-/gbpB- | |
| Strain Summary | gbpA/gbpB double null mutant | |
| Strain Descriptor | gbpA-/gbpB- | |
| Systematic Name | DBS0235992 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pcr pruducts with selection cassettes | |
| Associated Gene | pdeD pdeE | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1,bsR,pdeD-[gbpA-bsr],pdeE-[gbpB-pyr], pyr5-6-[pRG24],ura+ | |
| Phenotype | axenic,blasticidin resistant, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Identification of four candidate cGMP targets in Dictyostelium. Proc Natl Acad Sci U S A (2002) 99(10) 6749-54 [PubMed ID = 12011437] [RRC reference] Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ. A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. EMBO J (2002) 21(17) 4560-70 [PubMed ID = 12198158] [RRC reference] Bosgraaf L, Russcher H, Snippe H, Bader S, Wind J, Van Haastert PJ. Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium. Mol Biol Cell (2002) 13(11) 3878-89 [PubMed ID = 12429832] [RRC reference] |
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| Other Information | ||
| Depositor | Satoshi Sawai | |
| Position | ||
| Source | DSC | |
| Use Condition | A | |
| DSC | DBS0235992 | |
| Remarks | ||
| Images | ||
| Sequence | ||
