Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00371 | |
| Strain Name | ART/AX2 | |
| Strain Summary | AX2 cells expressing actin-thymosin fusion protein (ART) | |
| Strain Descriptor | ||
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 | |
| Mutagenesis Method | extrachromosomal_plasmid | |
| Mutagenesis Detail | pTIKL ART wild | |
| Associated Gene | act15 | |
| Foreign Gene | neoR | |
| Genotype | ||
| Phenotype | G418 resistance, axenic | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Noguchi TQ, Kanzaki N, Ueno H, Hirose K, Uyeda TQ.
A novel system for expressing toxic actin mutants in Dictyostelium and purification and characterization of a dominant lethal yeast actin mutant. J Biol Chem (2007) 282(38) 27721-7 [PubMed ID = 17656358] [RRC reference] |
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| Other Information | ||
| Depositor | Taro Uyeda | |
| Position | ||
| Source | T. Noguchi | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||

