Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00404 | |
| Strain Name | c1-/c11-, CI-/CII- | |
| Strain Summary | ctxA/ctxB (cortexillin I/II) double null mutant | |
| Strain Descriptor | ctxA-/ctxB- | |
| Systematic Name | DBS0235599 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | CII- (DBS0235600) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | ctxA-neo | |
| Associated Gene | ctxA ctxB | |
| Foreign Gene | bsR, neoR | |
| Genotype | axeA2,axeB2,axeC2,bsR,ctxA-[ctxA-neo],neoR,ctxB-[ctxB-bsr] | |
| Phenotype | axenic, blasticidin resistant,multiple null mutant ,neomycin resistant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Faix J, Steinmetz M, Boves H, Kammerer RA, Lottspeich F, Mintert U, Murphy J, Stock A, Aebi U, Gerisch G.
Cortexillins, major determinants of cell shape and size, are actin-bundling proteins with a parallel coiled-coil tail. Cell (1996) 86(4) 631-42 [PubMed ID = 8752217] [RRC reference] Honda G, Saito N, Fujimori T, Hashimura H, Nakamura MJ, Nakajima A, Sawai S. Microtopographical guidance of macropinocytic signaling patches. Proc Natl Acad Sci U S A (2021) 118(50) [PubMed ID = 34876521] [RRC reference] Takebayashi K, Kamimura Y, Ueda M. Field model for multistate lateral diffusion of various transmembrane proteins observed in living Dictyostelium cells. J Cell Sci (2023) 136(4) [PubMed ID = 36655427] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | G. Gerisch | |
| Use Condition | A | |
| DSC | DBS0235599 | |
| Remarks | ||
| Images | ||
| Sequence | ||
