Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00405 | |
| Strain Name | PI3K1-5-, pikA2-/pikB2-/pikC2-/pikF1-/pikG2- | |
| Strain Summary | pikA2-/pikB2-/pikC2-/pikF1-/pikG2- quintuple PI3K mutant (allele number 1 denotes bsR cassette present; allele number 2 denotes removal of the bsR cassette, but still leaving one lox site). | |
| Strain Descriptor | pikA-/pikB-/pikC-/pikF-/pikG- | |
| Systematic Name | HM1200 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 (DBS0235521) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous deletion, pOH74, pOH47, pOH109R, pOH89, pOH88 | |
| Associated Gene | pikF pikC pikA pikG pikB | |
| Foreign Gene | bsR | |
| Genotype | axeA2, axeB2, axeC2, pikA2-[pOH74], pikB2-[pOH47], pikC2-[pOH109R], pikF1-[pOH89], pikG2-[pOH88], bsR | |
| Phenotype | blasticidin resistant, axenic, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Hoeller O, Kay RR.
Chemotaxis in the absence of PIP3 gradients. Curr Biol (2007) 17(9) 813-7 [PubMed ID = 17462897] [RRC reference] Honda G, Saito N, Fujimori T, Hashimura H, Nakamura MJ, Nakajima A, Sawai S. Microtopographical guidance of macropinocytic signaling patches. Proc Natl Acad Sci U S A (2021) 118(50) [PubMed ID = 34876521] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | R. Kay | |
| Use Condition | A | |
| DSC | DBS0252652 | |
| Remarks | ||
| Images | ||
| Sequence | ||
