株リソース - 詳細
| Request |
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|
|---|---|---|
| NBRP ID | S00408 | |
| Strain Name | sgkAOE, sgkA-OE | |
| Strain Summary | sgkA overexpressor, FLAG tagged | |
| Strain Descriptor | [act15]:sgkA:FLAG | |
| Systematic Name | DBS0237193, SA604 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX3-ORF+ | |
| Mutagenesis Method | extrachromosomal plasmid | |
| Mutagenesis Detail | exogenous mutation, pDXA3C-sgkA-FLAG | |
| Associated Gene | sgkA | |
| Foreign Gene | neoR, FLAG | |
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pRG24], [Ddp2-ORF-pyr5-6],ura+, [act15/sgkA:FLAG], neoR | |
| Phenotype | axenic, FLAG-tagged, mutant,neomycin resistant, overexpressor, | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Min J, Traynor D, Stegner AL, Zhang L, Hanigan MH, Alexander H, Alexander S.
Sphingosine kinase regulates the sensitivity of Dictyostelium discoideum cells to the anticancer drug cisplatin. Eukaryot Cell (2005) 4(1) 178-89 [PubMed ID = 15643073] [RRC文献情報] |
|
| Other Information | neomycinG418 10ug/ml | |
| Depositor | DSC | |
| Position | ||
| Source | S. Alexander | |
| Use Condition | A | |
| DSC | DBS0237193 | |
| Remarks | ||
| Images | ||
| Sequence | ||
