Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00409 | |
| Strain Name | dagC-, erk2-, HS174 | |
| Strain Summary | erkB (erk2/dagC) null strain (REMI recapitulant) | |
| Strain Descriptor | erkB- | |
| Systematic Name | HS174 | |
| Species | Dictyostelium discoideum | |
| Category | Other | |
| Parental Strain | HL330 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | p240 ClaI | |
| Associated Gene | erkB | |
| Foreign Gene | ||
| Genotype | axeA1,axeB1,axeC1,dagC-, pyr5-6-[pΔPYR-BglII],ura+ | |
| Phenotype | axenic, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Segall JE, Kuspa A, Shaulsky G, Ecke M, Maeda M, Gaskins C, Firtel RA, Loomis WF.
A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium. J Cell Biol (1995) 128(3) 405-13 [PubMed ID = 7844154] [RRC reference] Gaskins C, Clark AM, Aubry L, Segall JE, Firtel RA. The Dictyostelium MAP kinase ERK2 regulates multiple, independent developmental pathways. Genes Dev (1996) 10(1) 118-28 [PubMed ID = 8557190] [RRC reference] Tsujioka M, Yamamoto T, Thompson CR, Kay RR, Maeda M. Novel development rescuing factors (DRFs) secreted by the developing Dictyostelium cells, that are involved in the restoration of a mutant lacking MAP-kinase ERK2. Zoolog Sci (2004) 21(8) 829-34 [PubMed ID = 15333995] [RRC reference] |
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| Other Information | ||
| Depositor | Hidekazu Kuwayama | |
| Position | ||
| Source | DSC | |
| Use Condition | A | |
| DSC | DBS0236383 | |
| Remarks | ||
| Images | ||
| Sequence | ||
