株リソース - 詳細
| Request |
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|---|---|---|
| NBRP ID | S00412 | |
| Strain Name | DS211C, severin-null | |
| Strain Summary | a truncated version of severin (domains 2+3). See pmid 1591239,Severin knockout obtained by transformation of AX2 with DS211C, | |
| Strain Descriptor | sevA- | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous insertion, pDNeo2 -DS211C | |
| Associated Gene | sevA | |
| Foreign Gene | neoR | |
| Genotype | axeA2, axeB2, axeC2, sevA-[DS211C], neoR | |
| Phenotype | axenic,null mutant, neomycin resistant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Eichinger L, Schleicher M.
Characterization of actin- and lipid-binding domains in severin, a Ca(2+)-dependent F-actin fragmenting protein. Biochemistry (1992) 31(20) 4779-87 [PubMed ID = 1591239] [RRC文献情報] Fisher PR, Noegel AA, Fechheimer M, Rivero F, Prassler J, Gerisch G. Photosensory and thermosensory responses in Dictyostelium slugs are specifically impaired by absence of the F-actin cross-linking gelation factor (ABP-120). Curr Biol (1997) 7(11) 889-92 [PubMed ID = 9480045] [RRC文献情報] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | P.Fisher | |
| Use Condition | A | |
| DSC | DBS0235923 | |
| Remarks | ||
| Images | ||
| Sequence | ||
