株リソース - 詳細
Request |
|
|
---|---|---|
NBRP ID | S00415 | |
Strain Name | pI/II-minus, PII/PI | |
Strain Summary | proA and proB double knockout (proB is deletion, proA is antisense) | |
Strain Descriptor | [AS]proA/proB- | |
Systematic Name | DBS0236827 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | proB- (DBS0236826) | |
Mutagenesis Method | antisense | |
Mutagenesis Detail | pDexRH-pI-ascDNA | |
Associated Gene | proA proB | |
Foreign Gene | neoR | |
Genotype | axeA2, axeB2, axeC2, FLAG:proC,neoR,proA-[pDexRH-pI-ascDNA], proB-[pDexRH-pII-cDNA], | |
Phenotype | axenic,multiple mutant, neomycin resistant, underexpressor | |
Axenic Growth | Y | |
Viable Spore Formation | ||
References |
Haugwitz M, Noegel AA, Karakesisoglou J, Schleicher M.
Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development. Cell (1994) 79(2) 303-14 [PubMed ID = 7954798] [RRC文献情報] Janssen KP, Schleicher M. Dictyostelium discoideum: a genetic model system for the study of professional phagocytes. Profilin, phosphoinositides and the lmp gene family in Dictyostelium. Biochim Biophys Acta (2001) 1525(3) 228-33 [PubMed ID = 11257436] [RRC文献情報] |
|
Other Information | ||
Depositor | DSC | |
Position | ||
Source | M.Schleicher | |
Use Condition | A | |
DSC | DBS0236827 | |
Remarks | ||
Images | ||
Sequence |