Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00415 | |
| Strain Name | pI/II-minus, PII/PI | |
| Strain Summary | proA and proB double knockout (proB is deletion, proA is antisense) | |
| Strain Descriptor | [AS]proA/proB- | |
| Systematic Name | DBS0236827 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | proB- (DBS0236826) | |
| Mutagenesis Method | antisense | |
| Mutagenesis Detail | pDexRH-pI-ascDNA | |
| Associated Gene | proA proB | |
| Foreign Gene | neoR | |
| Genotype | axeA2, axeB2, axeC2, FLAG:proC,neoR,proA-[pDexRH-pI-ascDNA], proB-[pDexRH-pII-cDNA], | |
| Phenotype | axenic,multiple mutant, neomycin resistant, underexpressor | |
| Axenic Growth | Y | |
| Viable Spore Formation | ||
| References |
Haugwitz M, Noegel AA, Karakesisoglou J, Schleicher M.
Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development. Cell (1994) 79(2) 303-14 [PubMed ID = 7954798] [RRC reference] Janssen KP, Schleicher M. Dictyostelium discoideum: a genetic model system for the study of professional phagocytes. Profilin, phosphoinositides and the lmp gene family in Dictyostelium. Biochim Biophys Acta (2001) 1525(3) 228-33 [PubMed ID = 11257436] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | M.Schleicher | |
| Use Condition | A | |
| DSC | DBS0236827 | |
| Remarks | ||
| Images | ||
| Sequence | ||
