Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00416 | |
| Strain Name | abpA-/abpC-/sevA-,HG1397 | |
| Strain Summary | abpA-/abpC-/sevA- triple null mutant (alpha-actinin, 120 kDa gelation factor, severin) produced by a combination of chemical mutagenesis, homologous recombination, and parasexual genetics. | |
| Strain Descriptor | abpA-/abpC-/sevA- | |
| Systematic Name | HG1397 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | ||
| Associated Gene | abpA abpC sevA | |
| Foreign Gene | neoR | |
| Genotype | axeA2, axeB2, axeC2,abp-tr(3), gelA1000, neoR, sevA1000 | |
| Phenotype | axenic,drug resistant, neomycin resistant, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Witke W, Schleicher M, Noegel AA.
Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins. Cell (1992) 68(1) 53-62 [PubMed ID = 1732064] [RRC reference] Witke W, Nellen W, Noegel A. Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. EMBO J (1987) 6(13) 4143-8 [PubMed ID = 2832153] [RRC reference] Schindl M, Wallraff E, Deubzer B, Witke W, Gerisch G, Sackmann E. Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy. Biophys J (1995) 68(3) 1177-90 [PubMed ID = 7756537] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | G.Gerisch | |
| Use Condition | A | |
| DSC | DBS0236169 | |
| Remarks | ||
| Images |
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| Sequence | ||
