株リソース - 詳細
Request |
|
|
---|---|---|
NBRP ID | S00416 | |
Strain Name | abpA-/abpC-/sevA-,HG1397 | |
Strain Summary | abpA-/abpC-/sevA- triple null mutant (alpha-actinin, 120 kDa gelation factor, severin) produced by a combination of chemical mutagenesis, homologous recombination, and parasexual genetics. | |
Strain Descriptor | abpA-/abpC-/sevA- | |
Systematic Name | HG1397 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | AX2 | |
Mutagenesis Method | homologous recombination | |
Mutagenesis Detail | ||
Associated Gene | abpA abpC sevA | |
Foreign Gene | neoR | |
Genotype | axeA2, axeB2, axeC2,abp-tr(3), gelA1000, neoR, sevA1000 | |
Phenotype | axenic,drug resistant, neomycin resistant, multiple null mutant | |
Axenic Growth | Y | |
Viable Spore Formation | NO | |
References |
Witke W, Schleicher M, Noegel AA.
Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins. Cell (1992) 68(1) 53-62 [PubMed ID = 1732064] [RRC文献情報] Witke W, Nellen W, Noegel A. Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. EMBO J (1987) 6(13) 4143-8 [PubMed ID = 2832153] [RRC文献情報] Schindl M, Wallraff E, Deubzer B, Witke W, Gerisch G, Sackmann E. Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy. Biophys J (1995) 68(3) 1177-90 [PubMed ID = 7756537] [RRC文献情報] |
|
Other Information | ||
Depositor | DSC | |
Position | ||
Source | G.Gerisch | |
Use Condition | A | |
DSC | DBS0236169 | |
Remarks | ||
Images | ||
Sequence |