Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00442 | |
| Strain Name | GFP-3Ala/HS1 | |
| Strain Summary | HS1 mhcA-null cells expressing GFP-mhcA with the 3 Ala mutation | |
| Strain Descriptor | mhcA-/[act15]:GFP-mhcA (3 Ala) | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | HS1 | |
| Mutagenesis Method | extrachromosomal_plasmid | |
| Mutagenesis Detail | pTIKL GFP 3 Ala myo | |
| Associated Gene | mhcA | |
| Foreign Gene | neoR, gfp | |
| Genotype | axeA1, axeB1, axeC1, neoR | |
| Phenotype | axenic, G418 resistant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK (poor) | |
| References |
Egelhoff TT, Brown SS, Spudich JA.
Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo. J Cell Biol (1991) 112(4) 677-88 [PubMed ID = 1899668] [RRC reference] Sabry JH, Moores SL, Ryan S, Zang JH, Spudich JA. Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells. Mol Biol Cell (1997) 8(12) 2605-15 [PubMed ID = 9398679] [RRC reference] |
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| Other Information | ||
| Depositor | Taro Uyeda | |
| Position | ||
| Source | depositor | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
