Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00447 | |
| Strain Name | atg5- | |
| Strain Summary | atg5 null mutant in DH1 | |
| Strain Descriptor | atg5- | |
| Systematic Name | HR75 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | atg5-KO (apg5-KO),endogenous mutation | |
| Associated Gene | atg5 | |
| Foreign Gene | bsR | |
| Genotype | axeA1,axeB1,axeC1,bsR,pyr5-6-[pRG24],ura-,atg5-[atg5-KO], | |
| Phenotype | axenic,, auxotroph, blasticidin resistant, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Otto GP, Wu MY, Kazgan N, Anderson OR, Kessin RH.
Macroautophagy is required for multicellular development of the social amoeba Dictyostelium discoideum. J Biol Chem (2003) 278(20) 17636-45 [PubMed ID = 12626495] [RRC reference] Otto GP, Wu MY, Clarke M, Lu H, Anderson OR, Hilbi H, Shuman HA, Kessin RH. Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum. Mol Microbiol (2004) 51(1) 63-72 [PubMed ID = 14651611] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | M. Wu (G. Otto) | |
| Use Condition | A | |
| DSC | DBS0236371 | |
| Remarks | ||
| Images | ||
| Sequence | ||
