Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00450 | |
| Strain Name | apg8-, atg8-, aut7 | |
| Strain Summary | atg8- macroautophagy null mutant | |
| Strain Descriptor | atg8- | |
| Systematic Name | HR141 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous mutation, atg8-KO | |
| Associated Gene | atg8 | |
| Foreign Gene | bsR | |
| Genotype | atg8-[atg8-KO],axeA1,axeB1,axeC1,bsR,pyr5-6-[pRG24],ura- | |
| Phenotype | auxotroph,blasticidin resistant, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Otto GP, Wu MY, Kazgan N, Anderson OR, Kessin RH.
Dictyostelium macroautophagy mutants vary in the severity of their developmental defects. J Biol Chem (2004) 279(15) 15621-9 [PubMed ID = 14736886] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | T. Tekinay | |
| Use Condition | A | |
| DSC | DBS0236340 | |
| Remarks | decreased fruiting body size on nitrocellulose filters; more severe effect on bacterial lawn | |
| Images | ||
| Sequence | ||
