Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00454 | |
| Strain Name | TL141 | |
| Strain Summary | csaA- (gp80) knockout in lagC- mutant (gp150). Similar to TL122. | |
| Strain Descriptor | csaA-/lagC- | |
| Systematic Name | TL141 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AK127 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | csaA-KO | |
| Associated Gene | tgrC1 csaA | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pΔPYR-BglII], ura+, lagC-[DIV2],csaA-[csaA-KO],bsR | |
| Phenotype | blasticidin resistant, axenic, multiple null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Dynes JL, Clark AM, Shaulsky G, Kuspa A, Loomis WF, Firtel RA.
LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium. Genes Dev (1994) 8(8) 948-58 [PubMed ID = 7926779] [RRC reference] Wang J, Hou L, Awrey D, Loomis WF, Firtel RA, Siu CH. The membrane glycoprotein gp150 is encoded by the lagC gene and mediates cell-cell adhesion by heterophilic binding during Dictyostelium development. Dev Biol (2000) 227(2) 734-45 [PubMed ID = 11071787] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | B. Loomis | |
| Use Condition | A | |
| DSC | DBS0236989 | |
| Remarks | ||
| Images | ||
| Sequence | ||
