Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00457 | |
| Strain Name | dnmA-null, dnmA-KO, Dnmt2 | |
| Strain Summary | DNA methyltransferase null mutant in AX2 | |
| Strain Descriptor | dnmA- | |
| Systematic Name | DBS0235917 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2-214 (DBS0235535) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | dnmA-bsr | |
| Associated Gene | dnmA | |
| Foreign Gene | bsR | |
| Genotype | axeA2,axeB2,axeC2,bsR,dnmA-[dnmA-bsr] | |
| Phenotype | axenic, blasticidin resistant, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Martens H, Novotny J, Oberstrass J, Steck TL, Postlethwait P, Nellen W.
RNAi in Dictyostelium: the role of RNA-directed RNA polymerases and double-stranded RNase. Mol Biol Cell (2002) 13(2) 445-53 [PubMed ID = 11854403] [RRC reference] Kuhlmann M, Borisova BE, Kaller M, Larsson P, Stach D, Na J, Eichinger L, Lyko F, Ambros V, Söderbom F, Hammann C, Nellen W. Silencing of retrotransposons in Dictyostelium by DNA methylation and RNAi. Nucleic Acids Res (2005) 33(19) 6405-17 [PubMed ID = 16282589] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | W.Nellen | |
| Use Condition | A | |
| DSC | DBS0235917 | |
| Remarks | ||
| Images | ||
| Sequence | ||
