Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S00474 | |
| Strain Name | calnexin-GFP, [act15]:cnx:GF | |
| Strain Summary | calnexin-GFP fusion | |
| Strain Descriptor | [act15]:cnxA:GFP | |
| Systematic Name | HG1738 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2-214 (DBS0235534) | |
| Mutagenesis Method | random insertion | |
| Mutagenesis Detail | exogenous mutation, act15/cnxA-RSSSKLK-gfp(S65T) in pDEXRH(G418) | |
| Associated Gene | cnxA | |
| Foreign Gene | neoR, gfp | |
| Genotype | axeA2,axeB2,axeC2,[calnexin-GFP in pDEXRH],neoR | |
| Phenotype | neomycin resistant, GFP marked, drug resistant, axenic | |
| Axenic Growth | Y | |
| Viable Spore Formation | ||
| References |
Müller-Taubenberger A, Lupas AN, Li H, Ecke M, Simmeth E, Gerisch G.
Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis. EMBO J (2001) 20(23) 6772-82 [PubMed ID = 11726513] [RRC reference] |
|
| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | A.Taubenberger | |
| Use Condition | A | |
| DSC | DBS0236184 | |
| Remarks | ||
| Images | ||
| Sequence | ||
