Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00476 | |
| Strain Name | AX2-N2-gfp, vat-gfp, ax-2-vat-gfp | |
| Strain Summary | AX2 cells expressing VatM-GFP | |
| Strain Descriptor | [act15]:vatM:GFP | |
| Systematic Name | DBS0235537 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 | |
| Mutagenesis Method | extrachromosomal_plasmid | |
| Mutagenesis Detail | exogenous mutation, pDXA/VatM-GFP | |
| Associated Gene | vatM | |
| Foreign Gene | neoR, gfp | |
| Genotype | axeA2, axeB2, axeC2, vatM-GFP, neoR | |
| Phenotype | neomycin resistant, GFP marked, overexpressor, axenic | |
| Axenic Growth | Y | |
| Viable Spore Formation | ||
| References |
Clarke M, Köhler J, Arana Q, Liu T, Heuser J, Gerisch G.
Dynamics of the vacuolar H(+)-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells. J Cell Sci (2002) 115(Pt 14) 2893-905 [PubMed ID = 12082150] [RRC reference] Gerisch G, Heuser J, Clarke M. Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium. Cell Biol Int (2002) 26(10) 845-52 [PubMed ID = 12421575] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | M.Clarke | |
| Use Condition | A | |
| DSC | DBS0235537 | |
| Remarks | ||
| Images | ||
| Sequence | ||
