Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S00483 | |
| Strain Name | AX4tgrB1-tgrC1-, tgrB1-/tgrC1- | |
| Strain Summary | tgrB1/tgrC1 double null strain with the pyr56 gene inserted in the tgrB1-C1 locus; the pyr56 gene is deleted from native locus and inserted in tgrB1 and tgrC1 locus | |
| Strain Descriptor | tgrB1-/tgrC1- | |
| Systematic Name | AK1540 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX4/ura- (AK1539) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous deletion with insertion, tgrB1/tgrC1-pyr56+ | |
| Associated Gene | tgrB1 tgrC1 | |
| Foreign Gene | ||
| Genotype | ||
| Phenotype | axenic | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Hirose S, Benabentos R, Ho HI, Kuspa A, Shaulsky G.
Self-recognition in social amoebae is mediated by allelic pairs of tiger genes. Science (2011) 333(6041) 467-70 [PubMed ID = 21700835] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | R. Benabentos | |
| Use Condition | A | |
| DSC | DBS0349731 | |
| Remarks | Rarely small fruit bodies are formed. it is difficult. | |
| Images | ||
| Sequence | ||
