Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S90109 | |
| Strain Name | RacF2-DN05-2 | |
| Strain Summary | overexpressor of dominant-negative RacF2 in racF2-(V12); resistant to blasticidin and neomycin; axenic | |
| Strain Descriptor | racF2-(V12)/[act15]:gfp:racF2(N17T) | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | V12KO#05 | |
| Mutagenesis Method | random insertion | |
| Mutagenesis Detail | pGFP:RacF2(N17T) | |
| Associated Gene | racF2 | |
| Foreign Gene | amp, bsR, neoR, gfp | |
| Genotype | racF2-,pRacF2-DN], neoR, gfp | |
| Phenotype | ||
| Axenic Growth | N | |
| Viable Spore Formation | OK | |
| References |
Muramoto T, Urushihara H.
Small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion. Dev Growth Differ (2006) 48(3) 199-208 [PubMed ID = 16573737] [RRC reference] |
|
| Other Information | racF2=GEG02 | |
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | Y. Furuya | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images |
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| Sequence | ||
