株リソース - 詳細

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NBRP ID S90110
Strain Name RacF2-DN11
Strain Summary overexpressor of dominant-negative RacF2 in racF2-(V12); resistant to blasticidin and neomycin
Strain Descriptor racF2-(V12)/[act15]:gfp:racF2(N17T)
Systematic Name racF2(N17T)
Species Dictyostelium discoideum
Category Recombinant
Parental Strain racF2-(V12)
Mutagenesis Method random insertion
Mutagenesis Detail pGFP:RacF2(N17T)
Associated Gene racF2 
Foreign Gene amp, bsR, neoR, gfp
Genotype racF2-,pRacF2-DN], neoR, gfp
Phenotype
Axenic Growth N
Viable Spore Formation OK
References Muramoto T, Urushihara H.
Small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion.
Dev Growth Differ  (2006)  48(3)   199-208  
[PubMed ID = 16573737]  [RRC文献情報
Other Information racF2=GEG02
Depositor Hideko Urushihara
Position
Source T. Muramoto
Use Condition A
DSC
Remarks
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