Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S90277 | |
| Strain Name | arpB+/arpB- | |
| Strain Summary | Arp2 heterozygous diploid arpB-/arpB+. (bsR cassette inserted in one copy of arpB) | |
| Strain Descriptor | arpB+/arpB- | |
| Systematic Name | DJK45 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DIR1 (DBS0235911) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous deletion, pRHI76 | |
| Associated Gene | arpB | |
| Foreign Gene | bsR | |
| Genotype | axeA1,axeB1,axeC1,pyr5-6-[pRG24],pyr5-6-[PYR5-6-SalI-linker],thy-[PYR5-6-THY1],ura+,thy+, arpB+/-[mycDdarpBpRHI76], bsR | |
| Phenotype | blasticidin resistant, axenic, mutant, heterozygous diploid | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Zaki M, King J, Fütterer K, Insall RH.
Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genet (2007) 8 28 [PubMed ID = 17553170] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | R. Insall | |
| Use Condition | A | |
| DSC | DBS0238408 | |
| Remarks | ||
| Images | ||
| Sequence | ||
