株リソース - 詳細
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NBRP ID | S90277 | |
Strain Name | arpB+/arpB- | |
Strain Summary | Arp2 heterozygous diploid arpB-/arpB+. (bsR cassette inserted in one copy of arpB) | |
Strain Descriptor | arpB+/arpB- | |
Systematic Name | DJK45 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | DIR1 (DBS0235911) | |
Mutagenesis Method | homologous recombination | |
Mutagenesis Detail | endogenous deletion, pRHI76 | |
Associated Gene | arpB | |
Foreign Gene | bsR | |
Genotype | axeA1,axeB1,axeC1,pyr5-6-[pRG24],pyr5-6-[PYR5-6-SalI-linker],thy-[PYR5-6-THY1],ura+,thy+, arpB+/-[mycDdarpBpRHI76], bsR | |
Phenotype | blasticidin resistant, axenic, mutant, heterozygous diploid | |
Axenic Growth | Y | |
Viable Spore Formation | OK | |
References |
Zaki M, King J, Fütterer K, Insall RH.
Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genet (2007) 8 28 [PubMed ID = 17553170] [RRC文献情報] |
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Other Information | ||
Depositor | DSC | |
Source | R. Insall | |
Use Condition | A | |
DSC | DBS0238408 | |
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Sequence |