Strain - Detail
| Request |
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|
|---|---|---|
| NBRP ID | S90278 | |
| Strain Name | arpB-/[act15]:myc:arpB | |
| Strain Summary | arpB- mutant rescued with myc-tagged Dictyostelium arpB under the control of the act15 promoter. | |
| Strain Descriptor | arpB-/[act15]:myc:arpB | |
| Systematic Name | MZ10 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DMZ10 (DJK45+pJSK19) | |
| Mutagenesis Method | haploid segregant | |
| Mutagenesis Detail | ||
| Associated Gene | arpB | |
| Foreign Gene | bsR, neoR, myc | |
| Genotype | axeA1, axeB1, axeC1, pyr5-6-[pRG24], pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, arpB-, bsR, neoR, [act15/myc:arpB] | |
| Phenotype | neomycin resistant, Myc-tagged, blasticidin resistant, axenic, mutant, rescued | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Zaki M, King J, Fütterer K, Insall RH.
Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genet (2007) 8 28 [PubMed ID = 17553170] [RRC reference] Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL. Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium. J Biol Chem (2013) 288(4) 2464-74 [PubMed ID = 23223240] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | R. Insall | |
| Use Condition | A | |
| DSC | DBS0238410 | |
| Remarks | ||
| Images | ||
| Sequence | ||
