株リソース - 詳細
NBRP ID | S90278 | |
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Strain Name | arpB-/[act15]:myc:arpB | |
Strain Summary | arpB- mutant rescued with myc-tagged Dictyostelium arpB under the control of the act15 promoter. | |
Strain Descriptor | arpB-/[act15]:myc:arpB | |
Systematic Name | MZ10 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | DMZ10 (DJK45+pJSK19) | |
Mutagenesis Method | haploid segregant | |
Mutagenesis Detail | ||
Associated Gene | arpB | |
Foreign Gene | bsR, neoR, myc | |
Genotype | axeA1, axeB1, axeC1, pyr5-6-[pRG24], pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy-, arpB-, bsR, neoR, [act15/myc:arpB] | |
Phenotype | neomycin resistant, Myc-tagged, blasticidin resistant, axenic, mutant, rescued | |
Axenic Growth | Y | |
Viable Spore Formation | OK | |
References |
Zaki M, King J, Fütterer K, Insall RH.
Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genet (2007) 8 28 [PubMed ID = 17553170] [RRC文献情報] Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL. Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium. J Biol Chem (2013) 288(4) 2464-74 [PubMed ID = 23223240] [RRC文献情報] |
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Other Information | ||
Depositor | DSC | |
Source | R. Insall | |
Use Condition | A | |
DSC | DBS0238410 | |
Remarks | ||
Images | ||
Sequence |