株リソース - 詳細
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| NBRP ID | S90279 | |
| Strain Name | sgkA-, sgkAΔ, SA512 | |
| Strain Summary | sgkA null mutant. The SalI-XbaI fragment containing the sgkA gene from cDNA clone SLG-787 was cloned into pBluescript. The EcoRI site within the sgkA gene was replaced with a BamHI site into which the 1.4-kb bsr cassette was cloned. Ax4 cells were electroporated with the above vector, linearized with SalI, and selected for blasticidin resistance. The resulting sgkAΔ strain is SA576. | |
| Strain Descriptor | ||
| Systematic Name | SA576 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX4 (DBS0235549) | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | ||
| Associated Gene | sgkA | |
| Foreign Gene | bsR | |
| Genotype | axeA1, axeB1, axeC1,bsR, sgkA- | |
| Phenotype | axenic, blasticidin resistant,null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Min J, Traynor D, Stegner AL, Zhang L, Hanigan MH, Alexander H, Alexander S.
Sphingosine kinase regulates the sensitivity of Dictyostelium discoideum cells to the anticancer drug cisplatin. Eukaryot Cell (2005) 4(1) 178-89 [PubMed ID = 15643073] [RRC文献情報] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | S. Alexander | |
| Use Condition | A | |
| DSC | DBS0236931 | |
| Remarks | ||
| Images | ||
| Sequence | ||
