Strain - Detail
| Request |
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|
|---|---|---|
| NBRP ID | S90282 | |
| Strain Name | mfeA- | |
| Strain Summary | mfeA null mutant (peroxisomal multifunctional enzyme; MFE1)) | |
| Strain Descriptor | mfeA- | |
| Systematic Name | MFE1 null | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | mfeA-KO | |
| Associated Gene | mfeA | |
| Foreign Gene | bsR | |
| Genotype | axeA2,axeB2,axeC2,bsR,mfeA-[mfeA-KO] | |
| Phenotype | axenic,blasticidin resistant, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Matsuoka S, Saito T, Kuwayama H, Morita N, Ochiai H, Maeda M.
MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp. Eukaryot Cell (2003) 2(3) 638-45 [PubMed ID = 12796309] [RRC reference] Matsuoka S, Kuwayama H, Ikeno D, Oyama M, Maeda M. Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium. Dev Growth Differ (2004) 46(2) 195-9 [PubMed ID = 15066197] [RRC reference] |
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| Other Information | ||
| Depositor | Mineko Maeda | |
| Position | ||
| Source | DSC | |
| Use Condition | A | |
| DSC | DBS0236544 | |
| Remarks | ||
| Images | ||
| Sequence | ||
