Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S90283 | |
| Strain Name | gefB-ko, Rasgef B-null, RasgefB- | |
| Strain Summary | RasgefB-null mutant. Does not grow in liquid: trying to do so will suppress the phenotype! | |
| Strain Descriptor | gefB- | |
| Systematic Name | ||
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX3 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | endogenous mutation, gefB-bsr | |
| Associated Gene | gefB | |
| Foreign Gene | bsR | |
| Genotype | axeA1,axeB1,axeC1,bsR,gefB-[gefB-bsr] | |
| Phenotype | axenic,drug resistant, null mutant | |
| Axenic Growth | N | |
| Viable Spore Formation | OK | |
| References |
Wilkins A, Chubb JR, Insall RH.
A novel Dictyostelium RasGEF is required for normal endocytosis, cell motility and multicellular development. Curr Biol (2000) 10(22) 1427-37 [PubMed ID = 11102804] [RRC reference] Wilkins A, Szafranski K, Fraser DJ, Bakthavatsalam D, Müller R, Fisher PR, Glöckner G, Eichinger L, Noegel AA, Insall RH. The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles. Genome Biol (2005) 6(8) R68 [PubMed ID = 16086850] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | R. Insall | |
| Use Condition | A | |
| DSC | DBS0236009 | |
| Remarks | ||
| Images | ||
| Sequence | ||
