Strain - Detail
| Request |
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|
|---|---|---|
| NBRP ID | S90289 | |
| Strain Name | GFPArp3, GFP-Arp3 | |
| Strain Summary | AX2 cells expressing a GFP-Arp3 fusion construct | |
| Strain Descriptor | [act15]:GFP:arpC | |
| Systematic Name | DBS0236065 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | AX2-214 (DBS0235534) | |
| Mutagenesis Method | random insertion | |
| Mutagenesis Detail | exogenous mutation, Gfp-Arp3 in pDEXRH | |
| Associated Gene | arpC | |
| Foreign Gene | neoR, gfp | |
| Genotype | axeA2,axeB2,axeC2,[GFP(S65T)-Arp3], neoR | |
| Phenotype | GFP marked, neomycin resistant, axenic, mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Insall R, Müller-Taubenberger A, Machesky L, Köhler J, Simmeth E, Atkinson SJ, Weber I, Gerisch G.
Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis. Cell Motil Cytoskeleton (2001) 50(3) 115-28 [PubMed ID = 11807934] [RRC reference] Fujimoto K, Nakano K, Kuwayama H, Yumura S. Deletion of gmfA induces keratocyte-like migration in Dictyostelium. FEBS Open Bio (2022) 12(1) 306-319 [PubMed ID = 34855306] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | G. Gerisch | |
| Use Condition | A | |
| DSC | DBS0236065 | |
| Remarks | ||
| Images | ||
| Sequence | ||
