株リソース - 詳細
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NBRP ID | S90292 | |
Strain Name | pikA-/pikB-/pikC-/pikF-/pikG-/pikH- | |
Strain Summary | pikA2-/pikB2-/pikC2-/pikF2-/pikG2-/pikH1- sextuple PI3K mutant (allele number 1 denotes bsR cassette present; allele number 2 denotes removal of the bsR cassette, but still leaving one lox site). | |
Strain Descriptor | pikA-/pikB-/pikC-/pikF-/pikG-/pikH- | |
Systematic Name | HM1284 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | pikA-/pikB-/pikC/pikF-/pikG- (DBS0238246) | |
Mutagenesis Method | homologous recombination | |
Mutagenesis Detail | pOH47, pOH74, pOH88, pOH89, pOH109R, pOH129 | |
Associated Gene | pikF pikC pikA pikG pikB pikH | |
Foreign Gene | bsR | |
Genotype | axeA2, axeB2, axeC2, pikA2-[pOH74], pikB2-[pOH47], pikC2-[pOH109R], pikF2-[pOH89], pikG2-[pOH88], pikH1-[pOH129], bsR | |
Phenotype | axenic, blasticidin resistant, multiple null mutant | |
Axenic Growth | Y | |
Viable Spore Formation | NO | |
References |
Hoeller O, Kay RR.
Chemotaxis in the absence of PIP3 gradients. Curr Biol (2007) 17(9) 813-7 [PubMed ID = 17462897] [RRC文献情報] Peracino B, Balest A, Bozzaro S. Phosphoinositides differentially regulate bacterial uptake and Nramp1-induced resistance to Legionella infection in Dictyostelium. J Cell Sci (2010) 123(Pt 23) 4039-51 [PubMed ID = 21045112] [RRC文献情報] |
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Other Information | ||
Depositor | DSC | |
Position | ||
Source | R. Kay | |
Use Condition | A | |
DSC | DBS0252653 | |
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