株リソース - 詳細

Request
NBRP ID S90306
Strain Name AGHR, AGHR2
Strain Summary abpA-/abpC- (alpha-actinin, ABP120) double null mutant
Strain Descriptor abpA-/abpC-
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain abpA- (DBS0235459)
Mutagenesis Method
Mutagenesis Detail pUC19-pDeltaGF-phleomycinR
Associated Gene abpA  abpC 
Foreign Gene bleR, neoR
Genotype axeA2, axeB2, axeC2, abpA-, abpC-, neoR, phleoR
Phenotype axenic, multiple null mutant, neomycin resistant, phleomycin resistant
Axenic Growth Y
Viable Spore Formation OK
References Eichinger L, Köppel B, Noegel AA, Schleicher M, Schliwa M, Weijer K, Witke W, Janmey PA.
Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants.
Biophys J  (1996)  70(2)   1054-60  
[PubMed ID = 8789124]  [RRC文献情報
Rivero F, Köppel B, Peracino B, Bozzaro S, Siegert F, Weijer CJ, Schleicher M, Albrecht R, Noegel AA.
The role of the cortical cytoskeleton: F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development.
J Cell Sci  (1996)  109 ( Pt 11)   2679-91  
[PubMed ID = 8937986]  [RRC文献情報
Other Information
Depositor DSC
Position
Source A. Noegel
Use Condition A
DSC DBS0235456
Remarks
Images
Sequence
page-top