Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S90308 | |
| Strain Name | HPS400 | |
| Strain Summary | thymidine auxotroph (tester strain) (strain is not thy-) | |
| Strain Descriptor | HPS400 | |
| Systematic Name | HPS400 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | HPS83 | |
| Mutagenesis Method | chemical | |
| Mutagenesis Detail | MNNG | |
| Associated Gene | ||
| Foreign Gene | ||
| Genotype | axeA1, axeB1, axeC1, cycA1, tsgA1, acrD369, ebrB364, sprH351, manA1, tmpA600, thy- | |
| Phenotype | cycloheximide resistance, temperature conditional mutant, multiple mutant, axenic, thymidine auxotroph, mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Shizukuishi S, Taniguchi T, Shibata S, Nakamura R, Tsunemitsu A, Uesugi Y.
Purification and some properties of alpha-L-fucosidase isolated from Streptococcus sanguis. J Dent Res (1978) 57(11-12) 1028-35 [PubMed ID = 281337] [RRC reference] Podgorski G, Deering RA. Thymidine-requiring mutants of Dictyostelium discoideum. Mol Cell Biol (1984) 4(12) 2784-91 [PubMed ID = 6527688] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | K. Saxe | |
| Use Condition | A | |
| DSC | DBS0236313 | |
| Remarks | ||
| Images | ||
| Sequence | ||
