Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S90368 | |
| Strain Name | MCF8-5 | |
| Strain Summary | REMI mutant defective in macrocyst formation | |
| Strain Descriptor | (macA) | |
| Systematic Name | MCF8-5 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | KAX3 | |
| Mutagenesis Method | homologous insertion | |
| Mutagenesis Detail | pMCF8.0 | |
| Associated Gene | macA | |
| Foreign Gene | bsR | |
| Genotype | axeA2, axeB2, axeC2, macA-, bsr | |
| Phenotype | axenic, blasticidin resistance, no macrocysts, no cell fusion | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Araki Y, Shimizu HD, Saeki K, Okamoto M, Yamada L, Ishida K, Sawada H, Urushihara H.
A surface glycoprotein indispensable for gamete fusion in the social amoeba Dictyostelium discoideum. Eukaryot Cell (2012) 11(5) 638-44 [PubMed ID = 22389384] [RRC reference] |
|
| Other Information | ||
| Depositor | Hideko Urushihara | |
| Position | ||
| Source | H.D. Shimizu | |
| Use Condition | A | |
| DSC | ||
| Remarks | ||
| Images | ||
| Sequence | ||
