Strain - Detail
| Request |
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|---|---|---|
| NBRP ID | S90405 | |
| Strain Name | myc2, Galpha2-, Ga2-, myc-2, Gα2- | |
| Strain Summary | Gα2 null mutant | |
| Strain Descriptor | gpaB- | |
| Systematic Name | DBS0236575 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | DH1 | |
| Mutagenesis Method | homologous recombination | |
| Mutagenesis Detail | pMYC14B | |
| Associated Gene | gpaB | |
| Foreign Gene | none | |
| Genotype | axeA1,axeB1,axeC1,gpaB-[pMYC14B],pyr5-6-[pRG24],ura+ | |
| Phenotype | axenic, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | NO | |
| References |
Chen MY, Devreotes PN, Gundersen RE.
Serine 113 is the site of receptor-mediated phosphorylation of the Dictyostelium G protein alpha-subunit G alpha 2. J Biol Chem (1994) 269(33) 20925-30 [PubMed ID = 8063709] [RRC reference] Cha I, Lee SH, Jeon TJ. Chemoattractant-mediated Rap1 activation requires GPCR/G proteins. Mol Cells (2010) 30(6) 563-7 [PubMed ID = 21103944] [RRC reference] Lilly PJ, Devreotes PN. Chemoattractant and GTP gamma S-mediated stimulation of adenylyl cyclase in Dictyostelium requires translocation of CRAC to membranes. J Cell Biol (1995) 129(6) 1659-65 [PubMed ID = 7790361] [RRC reference] Gundersen RE. Phosphorylation of the G protein alpha-subunit, G alpha 2, of Dictyostelium discoideum requires a functional and activated G alpha 2. J Cell Biochem (1997) 66(2) 268-76 [PubMed ID = 9213227] [RRC reference] |
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| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | P. Devreotes | |
| Use Condition | A | |
| DSC | DBS0236575 | |
| Remarks | ||
| Images | ||
| Sequence | ||
