Strain - Detail
| Request |
 |
|
|---|---|---|
| NBRP ID | S90422 | |
| Strain Name | Ddnhe1-/DdNHE1, NHE-/NHE-HA, nhe1-/act15, nhe1-/[act15]:, nhe1-/[act15], nhel-/[act15] | |
| Strain Summary | nhe1-null mutant expressing HA-tagged nhe1 under the control of the constitutive act15 promoter. | |
| Strain Descriptor | nhe1-/[act15]:nhe1:HA | |
| Systematic Name | DBS0236605 | |
| Species | Dictyostelium discoideum | |
| Category | Recombinant | |
| Parental Strain | nhe1- | |
| Mutagenesis Method | random Insertion | |
| Mutagenesis Detail | exogenous mutation, pB17S | |
| Associated Gene | nhe1 | |
| Foreign Gene | bsR, neoR | |
| Genotype | axeA2, axeB2, axeC2, nhe1-, nhe1:HA, bsR, neoR | |
| Phenotype | neomycin resistant, HA-tagged, axenic, rescued, blasticidin resistant, null mutant | |
| Axenic Growth | Y | |
| Viable Spore Formation | OK | |
| References |
Patel H, Barber DL.
A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis. J Cell Biol (2005) 169(2) 321-9 [PubMed ID = 15851518] [RRC reference] |
|
| Other Information | ||
| Depositor | DSC | |
| Position | ||
| Source | Chang-Hoon Choi (Diane Barber) | |
| Use Condition | A | |
| DSC | DBS0236605 | |
| Remarks | ||
| Images | ||
| Sequence | ||
