株リソース - 詳細
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NBRP ID | S00050 | |
Strain Name | HS1 (mhcA-) | |
Strain Summary | myosin II-null | |
Strain Descriptor | mhcA- | |
Systematic Name | HS1 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | JH10 | |
Mutagenesis Method | homologous recombination | |
Mutagenesis Detail | pTZD1THY (5.8-kb XbaI/ScaI fragment) | |
Associated Gene | mhcA | |
Foreign Gene | ||
Genotype | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+,mhcA-[pTZD1THY] | |
Phenotype | axenic | |
Axenic Growth | Y | |
Viable Spore Formation | NO | |
References |
Ruppel KM, Uyeda TQ, Spudich JA.
Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin. J Biol Chem (1994) 269(29) 18773-80 [PubMed ID = 8034630] [RRC文献情報] |
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Other Information | ||
Depositor | Taro Uyeda | |
Position | ||
Source | J. Spudich | |
Use Condition | A | |
DSC | ||
Remarks | ||
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Sequence |