株リソース - 詳細
NBRP ID | S00251 | |
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Strain Name | rasGKO-IR15(AX2) | |
Strain Summary | rasG null in AX2; resistant to blasticidin; axenic | |
Strain Descriptor | rasG- | |
Systematic Name | IR15 | |
Species | Dictyostelium discoideum | |
Category | Recombinant | |
Parental Strain | Ax2 | |
Mutagenesis Method | homologous recombination | |
Mutagenesis Detail | a version of pBsr deltaBam marker (Adachi et al., 1994) with BamHI linkers into the singleBglII site of pRASG | |
Associated Gene | rasG | |
Foreign Gene | bsR | |
Genotype | axeA2, axeB2, axeC2, rasG::bsR | |
Phenotype | ||
Axenic Growth | Y | |
Viable Spore Formation | OK | |
References |
Tuxworth RI, Cheetham JL, Machesky LM, Spiegelmann GB, Weeks G, Insall RH.
Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. J Cell Biol (1997) 138(3) 605-14 [PubMed ID = 9245789] [RRC文献情報] Kamimura Y, Miyanaga Y, Ueda M. Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis. Proc Natl Acad Sci U S A (2016) 113(16) 4356-61 [PubMed ID = 27044073] [RRC文献情報] |
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Other Information | ||
Depositor | Hideko Urushihara | |
Source | depositor | |
Use Condition | A | |
DSC | ||
Remarks | ||
Images | ||
Sequence |