株リソース - 詳細

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NBRP ID S00251
Strain Name rasGKO-IR15(AX2)
Strain Summary rasG null in AX2; resistant to blasticidin; axenic
Strain Descriptor rasG-
Systematic Name IR15
Species Dictyostelium discoideum
Category Recombinant
Parental Strain Ax2
Mutagenesis Method homologous recombination
Mutagenesis Detail a version of pBsr deltaBam marker (Adachi et al., 1994) with BamHI linkers into the singleBglII site of pRASG
Associated Gene rasG 
Foreign Gene bsR
Genotype axeA2, axeB2, axeC2, rasG::bsR
Phenotype
Axenic Growth Y
Viable Spore Formation OK
References Tuxworth RI, Cheetham JL, Machesky LM, Spiegelmann GB, Weeks G, Insall RH.
Dictyostelium RasG is required for normal motility and cytokinesis, but not growth.
J Cell Biol.  (1997)  138(3)   605-14  
[PubMed ID = 9245789]  [RRC文献情報
Other Information
Depositor Hideko Urushihara
Source depositor
Use Condition A
DSC
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