株リソース - 詳細

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NBRP ID S90813
Strain Name sGC-GFP in mhcAΔ
Strain Summary sGC-GFP/mhcAΔ
Strain Descriptor mhcAΔ cells expressing sGC-GFP
Systematic Name
Species Dictyostelium discoideum
Category Recombinant
Parental Strain mhcAΔ(S00050)
Mutagenesis Method extrachromosomal plasmid
Mutagenesis Detail sGC-GFP was cloned into pMB74 vector
Associated Gene sgcA  mhcA 
Foreign Gene gfp, neoR
Genotype axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+,mhcA-[pTZD1THY], sGC-GFP[pMB74::neoR]
Phenotype development defect, G418 resistant
Axenic Growth Y
Viable Spore Formation N
References Tanabe Y, Kamimura Y, Ueda M.
Parallel signaling pathways regulate excitable dynamics differently to mediate pseudopod formation during eukaryotic chemotaxis.
J Cell Sci  (2018)  131(23)  
[PubMed ID = 30404836]  [RRC文献情報
Other Information
Depositor Yuki Tanabe
Position
Source depositor
Use Condition B1
DSC
Remarks
Images
Sequence
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