Plasmid Vector - 詳細

Request
NBRP ID G90455
Name pTM1290
Description Multiplex CRISPR/Cas9 knockout system CRISPR final destination vector
Accession
DSC-ID
Associated Gene(s)
Backbone Plasmid pBluescript II
Antibiotic Resistance ampr  neor 
References Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium.
Sci Rep  (2018)  8(1)   8471  
[PubMed ID = 29855514]  [RRC文献情報
Map Multiplex_CRISPR-Cas9_Assembly_System.pdf
Sequence pTM1290.txt
Depositor Tetsuya Muramoto
Source Depositor
Other Information Ideal for multiple gene knockouts See Fig. S3 for details of the vectors. The multiple tRNA-sgRNA modules of the second module vectors can be assembled into the pTM1290 vector by BsmBI Golden Gate reaction. The combined Multiple tRNA-sgRNA modules can be digested with XhoI and HindIII and then cloned into pTM1285 or appropriate CRISPR vector.
Use Condition B1
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