Plasmid Vector - 詳細

Request
NBRP ID G90458
Name pTM1288
Description Multiplex CRISPR/Cas9 knockout system tRNA-sgRNA entry vector 3, pEV3
Accession
DSC-ID
Associated Gene(s)
Backbone Plasmid p3HD from Platinum Gate TALEN Kit
Antibiotic Resistance ampr  neor 
References Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium.
Sci Rep  (2018)  8(1)   8471  
[PubMed ID = 29855514]  [RRC文献情報
Map Multiplex_CRISPR-Cas9_Assembly_System.pdf
Sequence
Depositor Tetsuya Muramoto
Source Depositor
Other Information Ideal for multiple gene knockouts See Fig. S3 for details of the vectors. Add AGCA to the 5′ end of the sense oligonucleotide and AAAC to the 5′ end of the antisense oligonucleotide for the Golden Gate reaction with BpiI.
Use Condition B1
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