D. discoideum cDNA - Detail

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NBRP ID G00317
Name SFH642
Synonym dds16c12 
Accession BJ388794  BJ402316 
Dictybase ID DDB0216282 
Gene nek2 
Contig U08411
Remarks OK
References Gräf R.
Maltose-binding protein as a fusion tag for the localization and purification of cloned proteins in Dictyostelium.
Anal. Biochem.  (2001)  289(2)   297-300  
[PubMed ID = 11161327]  [RRC reference
Gräf R.
DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers.
J. Cell. Sci.  (2002)  115(Pt 9)   1919-29  
[PubMed ID = 11956323]  [RRC reference
Urushihara, H., Morio, T., and Tanaka, Y.
The cDNA Sequencing Project.
In: Methods in Molecular Biology (eds. Eichinger, L. and Rivero, F)  (2006)  346   31-49   [RRC reference
Web Site http://togodb.biosciencedbc.jp/togodb/view/dicty_cdb_lib
Host E. coli, DH10B
Cloning Vector pME18SFL3
Antibiotic Resistance amp-r 
Use Condition A
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