Plasmid Vector - Detail

NBRP ID G90426
Name pTM1285
Description All-in-one CRISPR/Cas9 vector Cas9-NLS-GFP, tRNA-sgRNA (BpiI) Transient expression (Neo)
Associated Gene(s) SpCas9 
Backbone Plasmid pBluescript II
Antibiotic Resistance ampr, neor 
References Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium.
Sci Rep  (2018)  8(1)   8471  
[PubMed ID = 29855514]  [RRC reference
Ogasawara T, Watanabe J, Adachi R, Ono Y, Kamimura Y, Muramoto T.
CRISPR/Cas9-based genome-wide screening of Dictyostelium.
Sci Rep  (2022)  12(1)   11215  
[PubMed ID = 35780186]  [RRC reference
G√ľnther M, Reimer C, Herbst R, Kufs JE, Rautschek J, Ueberschaar N, Zhang S, Peschel G, Reimer L, Regestein L, Valiante V, Hillmann F, Stallforth P.
Yellow polyketide pigment suppresses premature hatching in social amoeba.
Proc Natl Acad Sci U S A  (2022)  119(43)   e2116122119  
[PubMed ID = 36252029]  [RRC reference
Nguyen LTS, Robinson DN.
The lectin Discoidin I acts in the cytoplasm to help assemble the contractile machinery.
J Cell Biol  (2022)  221(11)  
[PubMed ID = 36165849]  [RRC reference
Map 1285.pdf
Sequence pTM1285.txt
Depositor Tetsuya Muramoto
Source Depositor
Other Information Ideal for gene knockout
Use Condition B1