Plasmid Vector - Detail
Request |
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NBRP ID | G90455 | |
Name | pTM1290 | |
Description | Multiplex CRISPR/Cas9 knockout system CRISPR final destination vector | |
Accession | ||
DSC-ID | ||
Associated Gene(s) | ||
Backbone Plasmid | pBluescript II | |
Antibiotic Resistance | ampr neor | |
References |
Sekine R, Kawata T, Muramoto T.
CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium. Sci Rep (2018) 8(1) 8471 [PubMed ID = 29855514] [RRC reference] |
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Map | Multiplex_CRISPR-Cas9_Assembly_System.pdf | |
Sequence | pTM1290.txt | |
Depositor | Tetsuya Muramoto | |
Position | ||
Source | Depositor | |
Other Information | Ideal for multiple gene knockouts See Fig. S3 for details of the vectors. The multiple tRNA-sgRNA modules of the second module vectors can be assembled into the pTM1290 vector by BsmBI Golden Gate reaction. The combined Multiple tRNA-sgRNA modules can be digested with XhoI and HindIII and then cloned into pTM1285 or appropriate CRISPR vector. | |
Use Condition | B1 |